14.91M

Похожие презентации:

BC-5000/5150series Service Training Hematology Global Technical Support Department

BC-3600 plus Service Training

BC-6000 Trouble Shooting Sharing

Common Laboratory Tests

7.Liquid System_V2.0_EN

1.

BC-6800 Service Training
-Hydraulic System
Mindray Hematology TS Dept.

Who We Are
What we do –
develop, manufacture and market medical devices that cater to hospital
needs around the world
How we do it –
vertically integrated business model; R&D oriented operation; A value
player
Who we are today –
a Chinese market leader with growing international presence
2

3.

BC-6800 Hydraulic System
Outline
1. Components
2. Main Fluidic Assembly &
Application
3. Fluidic Diagram
4. Sequence
5.Discussion
6. Trouble-Shooting

4.

Liquid Overview
Throughput 125T/H Max Auto - CBC+DIFF)
gram
36parameter+11
Blood OV 150ul 、 AL 200ul 、 Pre-dilute 40uL
Diluent Open-Vial CBC+DIFF 40ml Autoloader CBC+DIFF 30ml
Total 8 modes:

5.

Liquid Overview
Throughput
CBC
DIFF
CBC
RET
CBC
NRBC
CBC
DIFF
RET
CBC
DIFF
NRBC
CBC
DIFF
RET
NRBC
RET
Abbrevi C
ation
CD
CR
CN
CDR
CND
CNDR
R
WB
(test/H)
125
125
90
125
90
125
90
90
PD
(test/H)
36
36
30
36
30
36
30
30
Mode
CBC
Body Fluid Mode
Throughput: 40 tests /hour
RET Channel
Output RET%, Research para. (*PLT-O 、 *RBC-O 、 *WBC-R)
PD Mode
Increase test time, to ensure accurate result.

6.

Liquid Overview
Asp. Blood Decompose
Mode
Function
NRBC
Blood Segment(uL)
20
WBC/
BASO
20
DIFF
RET
RBC
HGB
20
4
4
4
B-F
4000(D)
Reagent volume
980(L)
980(L)
980(L)
996(D)
1496(D)
996(D)
(uL)
WB(Normal)
2000(LD),
1000(LB)
20(F)
0
20(F)
20(F)
0
520(L)
20(FD)
Diluted Ratio
51
50
51
255
375
380
/
Measurement time
4
5
5
4
10
/
/
Measurement volume
34.14
42.675
42.675
6.4
7.82
/
/
Background time
/
/
/
1
2
*Note D: Diluent
L: Lyse
F: Fluorescence reagent
/

7.

Liquid Overview
Main Features
1. Metering Pump (Diaphragm Pump)
20uL metering pump unitization design (better than competitor)
Cancel 2mL,3mL multi-specification metering pump, and utilize 1mL metering pump mostly
instead
Add 8mL metering pump to prime FCM chamber
2. Shear valve
Cancel twice shearing for RBC/PLT, simply the SRV design.
3. Syringe
Use homemade ceramic plunger syringe, 250uL WBC sample syringe which can support Triple
sample count
Use the Diba connecter ,which is easy for quick replacement
4. Pre-heating Assembly
Use plate frame structure, improve heating efficiency

8.

Liquid Overview
Main Features
5. Reaction Bath
New designed structure for heating, mixing,
Fluorescence inlet port particular design, avoid residue,
Over flow port post-position design
6. Sample preparation
WBC sample :Utilize 1mL metering pump and consider time and fixed amount, cancel buffer tube,
Sheath impedance (RBC/PLT): Negative pressure to prepare sample

9.

1.Components
Main Components
Electromagnetic
Valve(92)
Syringe
SRV
Diaphragm Pump(DP)
Type
Q’ty
Mindray Homemade
Common
53
Mindray Homemade
Hi-pressure
10
Burket Valve
3 (2-way:2, 3-way:1)
LVM Vavle
5(3-way:3,2-way:2)
Drain Valve
1
SMC gas valve
22
100uL (RBC)
1
250uL (WBC)
1
2.5mL (Asp.)
1
3 plates with blood sensor
1
1mL
6
20uL
3
8mL
1
1.5mL
1
0.52mL
1

10.

1.Components
Inlet
Hi-Pressure Valve: Thick Frame
Inlet: Air
Filter
Outlet
LVM Valve
Burket Valve

11.

1.Components
Pinch Valve: 13 pcs (5mm & 8mm)
Check Valve: 4 pcs (Lyse Dispense & Pierce Needle Air drying)
Inlet
Check Valve
Outlet

12.

1.Components
Air Filter + Air Drier
Air Filter
Life:2 year
Connecter
Air Drier
Life:4 year

13.

1.Components
Sheath Filter
Life:5 year
Life:6 Months

14.

1.Components
Air waste Filter
Filter for water:
5 year

15.

1.Components
New added tubes used in tubing system
1.0FEP
Thick
Pharmed
Tube
Black
PVC
ID 2.0/1.0
3350
Tube

16.

1.Components
Diaphragm Pump
Spec
Number
Function
DP1
0.52ml
HGB Lyse
DP2
1ml
HGB Diluent
DP3
1.5ml
RBC Diluent
DP4
1ml
NRBC Lyse (Needs to be changed
every half a year)
DP5
1ml
BASO Lyse
DP6
1ml
RET Diluent
DP7
1ml
DIFF Lyse
DP8
1ml
Optical sample preparation
DP9
20ul
DIFF Fluorescence
DP10
20ul
RET Fluorescence
DP11
20ul
NRBC Fluorescence
DP12
8ml
FCM 8ml Prime

17.

1.Components
WC1 Chamber
SV31
SV30
SV29
SV32
Wipe block waste
SV33
WC2
SV34
SV09 Sheath
Impedance
Buffer bath

18.

2. Main Fluidic Assembly & Application
2.1 Sampling Assembly
2.2 Reservoir Chamber
2.3 Temp. Control Assembly
2.4 HGB System
2.5 WBC System
2.6 RBC System

19.

2. 1 Sampling assembly
2.1.1 Sample Probe+Syringe
2.1.2 Shear Valve

20.

2.1.1 Sample Probe + Syringe
The similar structure of OV and AL Probe Assembly as BC-5800,But redesign probe wipe-block , which can not be exchanged
*Auto-sampling tube (between probe & SRV)was fixed volume, which can not be cut at filed,
please apply the specific code for replacement
P/N: 801-3201-00044-00
Syringe :
RBC sample dispensing 100ul
WBC sample dispensing 250ul
Sample Aspiration 2.5ml.

21.

2.1.3 Shear Valve
Protect
cover
Photo sensor
outer plate
Middle plate
Inner plate

22.

2.1.3 Shear Valve
Sensor 1(Inlet,
inner side)
Sensor 2(Outlet,
outer side)

23.

2.1.3 Shear Valve
Outer plate
Middle
plate
Inner plate

24.

2.1.3 Shear Valve-Outer plate

25.

2.1.3 Shear Valve-Middle plate

26.

2.1.3 Shear Valve-Inner plate

27.

2.1.3 Shear Valve

28.

2.1.3 Shear Valve-Blood Sensor

29.

2.1.3 Shear Valve-Blood Sensor
Blood
Blank
Sensor 1 blood signal
Detect principle
Blank
Sensor 2 blood signal
Pierce
needle
SRV front end
tube
SRV front
sensor
SRV
1
SRV
2
Syringe
SRV rear end
tube
SRV rear
sensor
SRV
connection
tube
SRV fixedvolume tube

30.

2.1.3 Shear Valve-Blood Sensor
Design point
I.
Sensor DA
II.
Sensor self-test
III.
Background check—Remove dirty &
bubble interference
IV.
Blood spark—skip over bubble
V.
Bubble identification
VI.
Problem report

31.

2.1.3 Shear Valve
Principle
Channel
blood volume
RBC
4uL
HGB
4uL
RET
4uL
BASO
20uL
DIFF
20uL
NRBC
20uL

32.

2.1.3 Shear Valve

33.

2.1.3 Shear Valve
DP
NRBC
BASO
DIFF
RET
HGB
RBC
OVSampling
AL-sampling
Shearing

34.

2. 3 Temp. Control Assembly
2.3.1 Pre-Heating Assembly
2.3.2 Reaction Bath Temp.
2.3.3 Sheath Temp.
2.3.4 Diluent Temp.
2.3.5 Flow Cell & Optical Assembly
Temp.

35.

2. 3 Temp. Control Assembly
Object
Target
Alarm
Range
Power
Source
Switch
Reaction
Bath
42℃
±3℃
70W/8.23Ω
AC24V
70℃
42~55℃
±3℃
85W/6.78Ω
AC24V
70℃
Diluent
25℃
/
50W/11.52Ω
DC24V
50℃
Flow Cell
32℃
+4/-3℃
10W/54.9Ω
AC24V
50℃
Sheath
35~40℃
±3℃
55W/10.47Ω
AC24V
50℃
Optic
Assembly
32℃
+4/-2℃
78W/7.3Ω
AC24V
50℃
Pre-Heating
Assembly

36.

2. 3.1 Pre-heating Assembly
2-way valve
Function: Pre-heating all
reagents except fluorescence
reagents.
Outlet
Target Temp. Range: 42~55°C
D
I
L
U
E
N
T
Accuracy: Target +/- 0.5°C
Time Limit: 540s Max. from
startup
Inlet

37.

2. 3.1 Pre-heating Assembly
Dissection: Temp. Part ; Mechanical Part ; Liquid Part

38.

2. 3.1 Pre-heating Assembly
Common Problem
Temp. Error
Assembly can not heat
Assembly leaking
Check drive board , temp. switch , temp. sensor , heating membrane.
Note: Temp. switch , temp. sensor and heating membrane cannot be
changed partially. If problem is caused by any one of them, change
the whole assembly.

39.

2. 3.2 Reaction Bath Temp.
Function: Provide the reaction
condition and place for WBC Diff
sample
Target Temp. Range: 39-45°C
1
2
Accuracy: Target +/- 0.5°C
Time Limit: 540s Max. from startup
3

40.

2. 3.2 Reaction Bath Temp.
Dissection: Temp. Control Module
Maintenance
1. Auto Cleaning.
2. Check No.8 port , unclog
with special tiny needle in
case.
3. Check if any leak.

41.

2. 3.2 Reaction Bath Temp.
Dissection: Mixing module
Step motor
Mixing Bar
Velocimeter tray
Belt
Clip
Maintenance
Upper
board
1. Lubricate.
photocoupler
Side board
2. Check any motor error
3. Check photo sensor.
Belt
pulley
Clip
Tightener
Quil
Down
board

42.

2. 3.3 Sheath Temp.
Function: Pre-heating the sheath
flow to make sure the optical
characteristic stable when laser
scattering
Target Temp. Range: 32~43°C
Accuracy: Target +/- 0.5°C
Time Limit: 540s Max. from startup

43.

2. 3.3 Sheath Temp.
Dissection
No. 5: Temp. Sensor
No. 6: Temp. Switch
No.12:Heating Membrane

44.

2. 3.4 Diluent Temp.
Function:
Provide Constant temp. in
case HGB/ RBC result drop
and improve MCV Calibration
while temp. sharply
fluctuating
Target Temp. Range: 12-42°C
Cycle 28.8s
Time Limit: 540s Max. from
startup
Inlet

45.

2. 3.4 Diluent Temp.
Outlet
Inlet
Heater

46.

2. 3.5 Flow Cell & Optical Assembly Temp.
Flow Cell Temp.
Optical Whole
Assembly
Temp. Sensor
Note: This is introduced in Optical System in details
Temp.Switch

47.

2. 4 HGB System
HGB Channel Principle
Fe 2+
Fe 2+
Fe
Fe 2+
Fe 2+
anionic surface active agent
1 、 Anionic surface active agent breaks down red blood cells
and white blood cells, and releases hemoglobin 。
2 、 Wave length 534nm 。
non-cyanide lyse
anionic surface active agent
HiCN
Fe
3+
Fe
3+
Fe
3+
3+

48.

2. 4 HGB System
New designed HGB Assembly
Splash-out proof design
Prevent fragmentary
bubble
Prevent Contamination
Easy- Mixing
Easy- Cleaning
Fixed Dispenser
Assembly- foolproof

49.

2. 4 HGB System
Light Source
Receiver
New Collimation Lens for LED light
Bigger receiving Area for HGB signal

50.

2. 4 HGB System
HGB Bath
1ml Metering
Pump
0.5mm Bubble
Tube
1.0FEP Sampling
Tube

51.

2. 4 HGB System
Diluent DP
Lyse DP
Shear blood
Asp. Blood
HGB Test Sequance
1.Drain
2.Prime DIL
2.Blank voltage (diluent)
3.Drain
4.Prime DIL、Blood、LH
5.Bubble Mixing
6.HGB sample voltage
7.Drain
8.Prime DIL
HGB
Bath
Bubble
Mixing
Drain
Bath

52.

2. 4 HGB System-Test Cycle
1 step
Drain the residue
and prepare the
diluent
st
DP2 Prepare
SRV
Drain residue

53.

2. 4 HGB System-Test Cycle
2nd step: Add diluent, and test
background
Add Diluent
Test
background

54.

2. 4 HGB System-Test Cycle
3rd,Drain residue,
prepare sample
and LH lyse
DP2 Ready
DP1 Ready
Drain again

55.

2. 4 HGB System-Test Cycle
4th Step, Add HGB
sample and read
voltage
Diluent and blood
into bath together
Add lyse
Bubble mixing

56.

2. 4 HGB System-Test Cycle
5th Step,
Prepare diluent
and drain waste
DP2 Ready
Drain waste

57.

2. 4 HGB System-Test Cycle
Add diluent into HGB bath
6th, Step: add second time
diluent (2mL) soaking
HGB bath

58.

2. 5 HGB System
* Notes for bubble tube replacement
The end of Teflon tube should
be out of three-way connecter
about 4mm.

59.

2. 5 HGB System
Main Problem Analysis
HGB Abnormal
HGB Bubble abnormal-Check air
bubble tube GV71 is good or
not;ID0.5mm teflon tube is
broken ,disconnection or not;
Prime&
Diluent
Drain Blank Volt
Drain
Drain
Sample
Voltage
No
bubble
Add
reagent and
Sample
mixing

60.

2. 5 HGB System
HGB Common problem
1.HGB Blank error
a. Check if the bath primed with diluent, if the liquid level is enough and no
bubble
b. Check if the diluent was changed before or not?
c. Bath?
d. HGB gain?
e. HGB unit?
2.Bubble abnormal
a. Check any block for bubble way.
b. Check the relative valve GV71 is working ok or not ?
c. Do you change the bubble tube correctly?

61.

2. 5 WBC System
Main components
Flow Cell Assembly
WBC Bath
250ul Syringe
Metering Pump
Sheath Pre-heating

62.

2. 5 WBC System—NRBC Test Cycle
1.Drain All Bath
2.Prime 1.0mL Lyse
(LN,LB,LD), Diluent
(DR)into relative bath.
Then drain empty again
for ready

63.

2. 5 WBC System—NRBC Test Cycle
All Bath will be dispensed
with blood, lyse and
fluorescence dye into
baths.

64.

2. 5 WBC System—NRBC Test Cycle
DP8 go ready for sample
Slow sheath open
V27 auxiliary dispense
250uL syringe work
Quick Sheath open
NRBC Test

65.

2. 5 WBC System—NRBC Test Cycle
1.
2.
3.
4.
5.
Slow sheath flush
probe and clean
flow cell
Next tests for Diff and Baso with similar steps
65
NRBC Slow
sheath to clean Flow
cell ,and flush sample
probe,
Open SV20 to flush
Flow cell.
DP12 、 PV14 、 SV55
open and prime FCM
chamber.
SV54 open and prime
DIL chamber
WC2 drain waste to
WC1

66.

2. 5 WBC System—NRBC Test Cycle
NRBC bath and tube clean
1.Sample tube clean with
SV28 PV05 open.
2.Open SV13 、 PV3 and
prime diluent 1.2mL
for soaking NRBC
bath for next
channels ready.
The channel test
sequence:
NRBC--BASO--DIFF--RET
Without heating and high
flux

67.

2. 5 WBC System—RET Test Cycle
1. RET Channel use
DP8for sample
ready
2. Slow sheath
3. V27 open for
dispense sample by
syringe
4. RET without Quick
sheath

68.

2. 5 WBC System—Clean
Finish optical test
Open SV19 、 SV20 、
SV45 to clean flow
cell outlet tube
Slow sheath-Flush
and clean flow cell
Slow sheath to clean
flow cell

69.

2. 5 WBC System—Reset without next test
1. Reset clean without
next test
2. WBC baths soak
with 1.2ml diluent
3. WBC baths and
tubing clean
4. FCM DIL bath
prime

70.

2. 5 WBC System—CBC Mode
T241-J1-P11
DP02
1.0mL
T146
T183
C58
C55
C45
SV22
SV39
VAC
70KPa
DP03
1.5mL
GV59
SV41
T439
T253
WC1-B
T315
T251
70KPa
C67
T207
C68
T323
GF1
T252
SV53
T205
T258
PV13/GV89
T254
C69
T204
WC1-I
WC1
SV31
C70
T202
WC1-S
WC1-A
T255
T256
-40KPa
C71
TC1
DP01
0.52mL
T201
C72
C73
T250
废液接头
WC1-H
GV71
T208
70KPa
废液桶
稀释液桶
T12
T10
RET试剂桶
T9
DIFF试剂桶
T8
BASO试剂桶
T441
VAC
70
T20
70KPa
VAC
T16
T131
T11
P26-T5-P27
P24-T4-P25
P22-T3-P23
P18-T1-P19
P20-T2-P21
T7
NRBC试剂桶
T6
HGB试剂桶
T32
T39
C151
C149
C148
T443
T27
VAC
70
T200
C64
WC1-O
WC1-G
GV48
GV57
DIL
J72-T172-J73
C65
T134
WC1-N
WC1-D
J79-T17-J82-P10
J80-T206-P1
T135
C63
T445
N
T133
SV21
SV24
T177
T145
C56
T173
T128
70
VAC
70
B
GV101
GV102
J65-T184
C66
T442
C155
C153
CV4
CV3
T38
D
分血阀运动汽缸
J75-T171
J74-T170
GV58
T449
T45
T450
T19
T15
T151-J86
CL-A
FCM
J52-T42-J53
J54-T35-J55
J71-T29-J56
RET
DIFF
J57-T22-J58
T31
R
T132
T180
J64-T198
VAC
70KPa
T176
WC1-C
WC1-R
SV23
C53
T175
C164
WC1-K
C74
GV60
DP04
1mL
SV35
P_SYRINGE
2.5mL
C170
T179
J67-T141
T195
开放拭子
GV61
DP05
1mL
HGB池
C158
C159
C165
WC1-H
C60
T174
T203
T129
C57
C166
WC1-RC
DP07
1mL
C130 T199-J78-P2
T185
WC1-G
C50
T197
SV37 T196
SV40
T113
SCI
T148
C49
C52
C129
WC2-1
SV08
C54
开放采样针
J39-T87-P31
T86
T194
SV38
J76-T127-J77
C38
T110
T193
PV12/GV88
WC1-K
T112
C59
T181
T111
C37
T189
C51
T182
T89
C42
T191
C40
C61
SV36
T192
C46
R_SYRINGE
100μ L
C41
J62-T167-J63
C48
T178
T92
SV07
T209
T115
SV30
T190
J68-T140-J69
T139
T137
T150
T95
T93
C62
CV1
T188
T106
T90
GV70
T138
T107
J66-T142
PV06/GV84
T149
T109
T97
穿刺针
T143
PV11/GV87
SV49
GV63
GV62
穿刺拭子
WC1-C
T147
SV01
C39
C44
RBC预
混池
T88-J36
鞘流阻抗
池
J34-T126
T108
T94
SV05
C34
T96
T91
SV03
ISW
ISU
T116-J35
T249 PV16/GV99
SV02
SV04
C36
T79
T104
T144
T99
T105
J42-T98-J43
WC1-I
TS
70KPa
T102
T186-P8
T187
T103
C128
T154
T156-J100
C160
C167
WC1-F
DP06
1mL
Open
T101
T120
C47
CV2
T25
Open
J46-T117-J47
T100
WC1-RC
C43
T169
T168
PV07/GV85
J30-T118-J31
T119
C127
SV42
T153
T155
NRBC
BASO
DIFF
RET
HGB
RBC
C161
C168
C89
WC1-A
WC1-F
Ta-P7
SV09
T121
自动进样
采样管
SV25
GV74
T130
C162
Diluent will be
primed into
RET 、 NRBC 、 DIFF
bath instead of lyse
C150
SV50
T33
T136
T152-J37
C152
C163
T440
T444
SV52
SV51
CL-A
GV75
T312
BASO
分血阀
C154
T447
T40
VAC
70
T157
C156
T311
C33
T446
T21 T34 T28 T41
C171
Open
RH
J20-T84-J33
T83
WC2
T257
250
VAC
T163
T165
J51-T36-J61
SV28
SV27
J50-T23-J60
C15
SV14
SV15
SV16
T210
T215
WC1-N
T166
C19
T220
J59-T43-J97
T81
C31
C24
T164
J44-T162-J45
C22
C23
J40-T158-J41
C138
C139
C140
C141
C142
C143
C169
T62
T159
C21
T214 SV32
C18
WC1-D
T230
WC1-R
T82-J21
T219 SV33
C17
WC1-B
T80
T229SV29
C16
T225
LF2
T58
DIL
T125
PV05/GV83
T224 SV34
T60
C32
J48-T160-J49-P9
T57
T85
SCI
T37-P5
T161
C20
SV46
FCM
SV47
T59
T211
T54
GV56
C25
SV13
T53
SV55
T114
T124
C132
T46C133
T49C134
T18C135
T13C136
T14 C137
T26
T52
T24-P3
T44-P6
T30-P4
T248
C5
J28-T213-J29
PV01/GV79
T216
C4
DH
LF1
T48
DP12
8mL
NRBC
T221
T212
C27
SCI
T61
SHEATH
T232-J14
C3
T217
溢流接头
T263
T453
TC3
T123
T448
PV14/GV97
GV73
DIL
C30
C29
T47
T51
SV54
160
T262
DIFF
T259
C2
PV02/GV80
PV03/GV81
T226
T231
S_SYRINGE
250μ L
DP08
1mL
T227
T264
J26-T218-J27
T222
WC1-S
T266
J22-T228-J23
T68
T265
J24-T223-J25
C14
T63
SV17
C11
T67
GV44
C28
T50
T55
FCM
SH
C1
GV68
BASO
WBC池
SV18
J17-T233-J16
SV26
NRBC
J8-T64-J9
PV04/GV82
T65
T69
T236
WC1-O
J18-T234-J19
T235
C13
70KPa
VAC
T239
T238
T237
J12-T73-J13
T66
C10
J10-T71-J11
WC2-1
SV19
FC
PV15/GV98
C9
T72
SV20
SV45
C12
RET
GV66
T70
T56
40
T260
T261
P30-J15
P29-J7
NRBC荧光染料
C8
T74
DP11
20uL
NRBC
C26
VAC
70
J85-T246-P34
J84-T243-P33
T76
RET
J4-T75-J5
J89-T452-J90
J93-T77-J94
C7
GV65
T247-J3-P13
SV12
Open
T78
DP10
20uL
T244-J2-P12
P37
VAC
70
RET荧光染料
GV64
SV11
J87-T451-J88
P28-J6
DP09
20uL
DIFF
P36
VAC
70
C6
SV10
VAC
70
DIFF荧光染料
J83-T240-P32
P35

71.

2. 6 RBC System
New RBC System-Sheath Impedance
(DC-Flow Method)
*New sheath impedance is improved
from electric impedance method, as
shown right pic: the sample flow will
be surrounded by front sheath fluidic
and forced to pass through aperture
one by one, and taken away from test
area quickly by back sheath fluid.
Electrodes in bath will detect Pulse
change when cell pass through
aperture which will reflect the cell
size.

72.

2. 6 RBC System
Blood4ul
PLT
RBC
M-68DS1.5ml
RBC
RBC/PLT Bath
1:375
Sheath Impedance
RBC/PLT Result
PLT

73.

2. 6 RBC System
Sheath Impedance Advantage
Better Data Collection ——Less irregular pulse, Decrease M wave more accurate
single wave detection, better histogram 。
Higher Speed——Single dilution (5800 dilution ratio 1 18000 6800 1 375);
Less Reagent——No Volumetric tube & Pre-mixing bath clean about 10ml less
diluent;
Reduce Clog;

74.

2. 6 RBC System

75.

2. 6 RBC System
Front Sheath
Back Sheath

76.

2. 6 RBC System
Clean

77.

2. 6 RBC System
New RBC System-Sheath Impedance
•Main parts
•Sample preparation system
•Sample detection system

78.

2. 6 RBC System-Main parts
SCI Chamber
Provide sheath fluidic for RBC
counting:
1. Directly supply front sheath
2. Supply sheath for ISU bath
3. Drive sheath for sample flow
4. Asp. Sheath for syringe

79.

2. 6 RBC System-Main parts
RBC bath
Provide RBC sample for RBC counting:
a. 4ul blood
b. 1.5ml diluent
c.1:375 ratio diluted sample

80.

2. 6 RBC System-Main parts
Sheath impedance front
panel assembly
Pinch Valve
ISU bath
Counting bath
ISW bath

81.

2. 6 RBC System-Main parts
ISU bath
. Isolated unit for back sheath
. provide driven pressure for fluidic
flow to clean rear bath.

82.

2. 6 RBC System-Main parts
ISW bath
.Collect the waste from counting
bath,
. Isolated chamber

83.

2. 6 RBC System-Main parts
Sheath Counting bath
Collect cell impedance signals

84.

2. 6 RBC System-Main parts
Sheath counting bath maintenance
1 、 Check any leaking of O–ring;
2 、 Check any deformation , damage of Electrodes;
3 、 Any block at aperture

85.

2. 6 RBC System-Main parts
Disassemble and re-assemble front sheath bath

86.

2. 6 RBC System-Main parts
Sheath Impedance assembly
.Shielding
. Negative pole grounding

87.

2. 6 RBC System-Main parts
Sheath Impedance assembly
•maintenance:
Check and clean any leaking from
sheath impedance assembly

88.

2. 6 RBC System-Test Cycle
Sheath Impedance Process
T311
GV75
Open
T130
T312
GV74
C33
Aspiration and
SV09
J30-T118-J31
T119
J46-T117-J47
Shear
C43
C127
T100
T101
2. Reaction and
T120
T103
TS
C47
C36
Mixing
T144
T79
T102
ISU
T249 PV16/GV99
T121
3. Sample ready
Impedance
SV02
C34
Bath
ISW
4. Auxiliary dispense
SV03
T96
SV04
5. Sample Count
SV01
T109
SV05
T107
C39
6. Background
T97
T92
T95
7. Cleaning
T106
T90
C40
T93
Main parts
SV07
C41
T110
RBC Impedance
T111
C37
T89
T112
C42
Bath
J39-T87-P31
T86
C38
SV08
RBC Pre bath
T113
100ul Syringe
GV84 normal Close
GV99 normal open
SV08,SV09 :LVM 2way SMC valve
T169
T115
T88-J36
T186-P8
NRBC
BASO
DIFF
RET
HGB
RBC
J65-T184
T183
C58
C55
T181
T188
T146
C46
T191
VAC
70KPa
T182
SV30
T190
WC1-RC
R_SYRINGE
100μL
T116-J35
J34-T126
T91
T94
WC1-G
PV06/GV84
C44
RBC
Premix
Bath
DIL
T187
T104
Open
分血阀
T108
T99
T105
J42-T98-J43
SCI
WC1-I
T168
1.
DP03
GV59 1.5mL

89.

2. 6 RBC System-Test Cycle
T311
GV75
Open
T130
T312
GV74
SV09
J30-T118-J31
T119
C43
C127
T169
T168
C33
J46-T117-J47
T100
SRV
T101
T120
T103
T95
Open
DIL
C58
C55
T146
C38
WC1-G
T113
T110
J39-T87-P31
T86
SV08
SV30
T190
C46
T191
WC1-RC
T111
C37
R_SYRINGE
100μL
SV07
T112
SCI
T183
T188
C40
T93
C42
J65-T184
T106
T90
T89
T186-P8
T181
T92
RBC
Premix
Bath
NRBC
BASO
DIFF
RET
HGB
RBC
VAC
70KPa
T182
T97
T107
T88-J36
T109
C44
T115
PV06/GV84
SV01
C39
C41
T187
Impedance
Bath
T116-J35
SV05
C34
T96
T94
SV04
ISW
ISU
J34-T126
T99
T105
SV02
SV03
C36
T79
T249 PV16/GV99
T91
WC1-I
J42-T98-J43
T121
T144
T104
T102
T108
C47
TS
DP03
GV59 1.5mL

90.

91.

Sheath Ready
Sheath & sample ready
鞘流阻抗液路结构图
Sample
Ready
Generate
sheath
Syringe
Ready

92.

Dispense sample for detection
鞘流阻抗液路结构图
Effective
sample
Measurement
Keep
sheath
Dispense

93.

94.

2. 6 RBC System-Test Cycle
T311
GV75
Open
T130
T312
GV74
SV09
J30-T118-J31
C43
C127
J46-T117-J47
T100
Clean
front bath
T101
T120
SRV
T103
T97
T107
T92
T95
WC1-G
DIL
Open
C55
Clean rear
bath
J39-T87-P31
T86
SV08
SV30
T190
C46
T191
WC1-RC
T113
T110
R_SYRINGE
100μL
T111
C37
C38
SCI
T183
C58
T146
SV07
T112
C42
J65-T184
T188
C40
T93
T89
T186-P8
T106
T90
C41
RBC
Premix
Bath
NRBC
BASO
DIFF
RET
HGB
RBC
T181
C39
T109
C44
T88-J36
SV01
T115
PV06/GV84
Soak bath
T187
Impedance
Bath
T116-J35
SV05
C34
T96
T94
SV04
ISW
Flush
Probe
ISU
J34-T126
T99
T105
SV02
SV03
C36
T79
T249 PV16/GV99
T91
WC1-I
J42-T98-J43
T121
T144
T104
T102
T108
C47
TS
VAC
70KPa
T182
T119
T169
T168
C33
DP03
GV59 1.5mL

95.

3. Fluidic Diagram
Reaction
Module
Fluorescenc
e
Module
DL Prime
Module
Reagent Detection
Module
Sheath
Heating
Pre-Heating
Module
WBC
Measuremen
t
Maintenance
Module
RBC
Module
SRV Module
Waste
Module
HGB
Module

96.

Main sequence
Dilution
NRBC FL 20uL
NRBC Lyse 1mL
20uL
Bath 1:51
DIFF Lyse 1mL
20uL
Bath 1:51
BASO Lyse 1mL
20uL
Bath 1:50
RET Diluent 1mL
4uL
Bath 1:255
Diluent 1mL
4uL
HGB Bath 1:375
Diluent 1.5mL
4uL
RBC Bath 1:375
SRV
DIFF FL 20uL
RET FL 20uL
HGB Lyse 0.5mL

97.

Main sequence-Sampling
1.Preparation
2.2.5mL syringe
Asp.
3.Optical coupler
detection
4.Clean (probe)

98.

Main sequence-SRV Dispense
1.SRV Switch
2. Drain Baths
3.Prime sample &
Reagent
4. Add FL 20ul
5.HGB Add Lyse
6.Mixing

99.

Main sequence-Measurement
WBC
1.DP8 Sample
Preparation
2.Start slow sheath
3.V27, Syringe dispense
4.Start quick sheath
RET Mode: NO
5.Measurement
RBC
1.SV09 start sample
preparation
2.Start front & rear
sheath
3.Syringe dispense
4.Measurement
Prime DIL FCM SCI
bath

100.

Main sequence-Clean & Drain
WBC
1.Clean reaction bath
2.Sample tube clean
3.Sheath tube , probe
Flush Flow cell
clean
RBC
1.RBC Bath Clean
2. Sample tube clean
3.Flush
4.Sample probe flush
5.ISW Bath Drain
Common part
Drain WC1 WC2
Bath

101.

Main sequence-Reset Clean
Reset Clean without
next sample
1.WBC soaking 1.2ml
diluent
2.WBC Bath & Tube
clean
3.RBC soaking 0.7ml
diluent
4.RBC Sample Tube
Clean Sample
probe Flush
5.FCM SCI Bath
Prime

102.

Maintenance sequence
Maintenance
Whole Device Probe Cleanser
Daily Shutdown Probe Cleanser
Auto-Protect Probe Cleanser
Probe Cleanser BASO
Probe Cleanser NRBC
SRV Probe Cleanser
Flow Cell Probe Cleanser
Aperture Probe Cleanser
HGB Bath Probe Cleanser

103.

Probe Cleanser Maintenance

104.

Maintenance Solution
Auto
Mai ntenance
Startup
Startup 1 af ter
shutdown normal l y
Startup 2 af ter
shutdown normal l y
Startup 1 af ter
shutdown normal l y
Startup 1 af ter
shutdown abnormal l y
Mai ntenanc
e Sol uti on
Mode
Swi tch
Standby
Sampl i ng Mode
swi tch
Bl ood mode
swi tch
Measurement
mode swi tch
Goto standby
Qui t f romshort
standby 1
Qui t f romshort
standby 2
Qui t f roml ong
standby
Common user &
admini strator
Startup af ter pack
End- user
Mai ntenance
Servi ce above
Probe
Cl eanser
Shutdown
Dai l y
shutdown
Ti mel y
shutdown
Mai ntenance on
threshol d 1( BASO
Pack up
Whol e Devi ce
Fl ow Cel l
SRV
Mai ntenance on
threshol d 2 NRBC
Aperture
HGB Bath
BASO Bath
NRBC Bath
Mai ntenance
i n need
Regent
Repl acement
Pri me
Di l uent
Drai n/Pri me
( chamber)
Cl ean
Fl ui di c
Reset
Uncl og
Di l uent
WBC Bath
Reagent Chamber
Uncl og
DI FF Lyse
DI FF Lyse
RBC i mpedance
Bath
Reacti on Bath
Fl ush
DI FF FL
DI FF FL
HGB Bath
Waste chamber
Zap
BASO Lyse
BASO Lyse
RBC Bath
Whol e Devi ce
RET
Di l uent
RET
Di l uent
RET FL
RET FL
NRBC Lyse
NRBC Lyse
SRV
NRBC FL
NRBC FL
OV Sampl i ng part
HGB Lyse
HGB Lyse
Auto Sampl i ng part
Remove Fl ow Cel l bubbl e
Fl ow Cel l Cl ean
Whol e Devi ce

105.

5. Discussion
How to check problem from
scatter gram?

106.

Trouble-shooting(Fluorescence clog in Diff)
Fresh blood
Calibrator

107.

Mixing DIFF
Mixed
No-Mix

108.

Fluorescence Volume DIFF
10uL
1#
2#
15uL
20uL
25uL

109.

Fluorescence Volume RET
10uL
1#
2#
15uL
20uL
25uL

110.

Fluorescence Volume NRBC
10uL
15uL
20uL
25uL

111.

Analysis
If the temp. is constant, the fluorescence intensity decrease,it means the
concentration of solution in bath is low.
Concentration is low caused by low fluorescence volume into bath.
Considering the mixing function is good or not?
If mixing is well, please check related inlet port clog for fluorescence reagent? Or
20uL Diaphragm pump problem, or tubing block reason?
Q: how to use sample special info. (WBC_D/WBC_B, WBC_N/WBC_B,
RBC_O/RBC_I) to check lyse injection volume?