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Practical pharmacology. Part 4. Dose response curve of acetylcholine
1.
Practical pharmacologyPart 4
2.
Dose response curve of acetylcholine3.
Organ bathDrum
Water bath
4. Conditions
Physiological solutionTemperature
Aeration
5. Our isolated organ
• Rabbit intestine• Tyrode‘s solution
• Temperature 37˚C
• Aeration by air pump
6.
7.
8.
AchAch
contraction
9.
10.
11.
12.
13.
Myogenic contraction0.05
wash
0.1
0.2
0.3
14.
Now we need to draw the dose response15.
5 cmPeak
5 cm
4 cm
3 cm
Response
0.05
Base line
0.1
0.2
0.3
16.
Important note0.05 ml
0.2 ml
0.1 ml
0.3 ml
Is volumes
We need to convert volumes into doses
?
17.
Conc. of ach.0.1 g%
0.1 g
100 ml
x
0.1 ml
18.
x =0.1 x 0.1
100
= 0.0001 g
= 0.1 mg
19.
Now draw your curve20.
Response (cm)7
6
x
5
x
4
3
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3
21.
Notes22.
Response (cm)7
6
x
5
x
4
3
x
x
2
1
This
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3
23.
Response (cm)7
x
6
5
x
4
3
x
x
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3
24.
Response (cm)7
x
6
5
x
4
3
x
x
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3