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BC-760_780_Product Training_EN_V1.0

1.

BC-760&780_Product Training
IVD Global Technical Support Department
Version: V1.0

Above and Beyond
B C - 7 0 0 S e r i e s · S F C u b e · H y b r i d C h a n n e l P LT · E a s y - W E S R
Excellent
Superior
Interactive
Performance
Functionality
Excellence
SF Cube fluorescent
Auto Hematology
technology
Analyzer with ESR
IMG/NRBC Reportable
Optical PLT-H in every CD
parameter
test
Accurate measurement of
Integrated RET & body
low WBC/PLT count
fluid analyzer
Floating screen
iHelp videos
Ease of maintenance

5.

Product model
Model BC-760[B] / BC-760[R] / BC-780[R]
Intended use It provides Complete Blood Count,
Leukocyte Differential, Hemoglobin Concentration
Measurement, Reticulocyte Measurement, Erythrocyte
Sedimentation Rate Measurement for blood samples, as
well as Body Fluid analysis.
Configuration difference
Model
Autoloader + Closed-Tube model
BC-700 Specification
Width 400mm
Size
Height 600mm
Depth 840mm
Weight
70.6kg
Sampling
mode
RET
ESR
BC-760[B]
/
BC-760[R]
BC-780[R]
Storage capacity

6.

Reagent(M-6)
Reagent
Model
BC-700[B]/BC-760[B]
HGB
M-6 LH Lyse
M-6 LD Lyse
DIFF
RET
BC-700[R]/BC-720[R]/
BC-760[R]/BC-780[R]
M-6 FD Dye
/
*M-6 DR Diluent
/
*M-6 FR Dye
Diluent
DS Diluent
Daily
Maintenance
Probe cleanser
ESR
ESR Cleanser
ESR Cleanser
(orange)

7.

C o n t r o l & C a l i br a to r
Name
Control
Level
Applicable model
Model
Parameters
Hematology Control
BR60
High, Normal, Low
BC-760[B]/BC-760[R]/
BC-780[R]
Hematology Control
BC-6D
High, Normal, Low
BC-760[B]/BC-760[R]/
BC-780[R]
Hematology Control
BC-RET
High, Normal, Low
BC-760[R]/BC-780[R]
RET series report parameters
Hematology Control
BC-BF
High, Normal, Low
BC-760[B]/BC-760[R]/
BC-780[R]
Body fluid sample test report
parameters
Calibrator
Model
Name
All blood sample test report
parameters except ESR
All blood sample test report
parameters except RET series
(include ESR)
Applicable model
Hematology Calibrator
SC-CAL PLUS
BC-760[B]/BC-760[R]/BC-780[R]
Hematology Calibrator
SC-CAL RET
BC-760[R]/BC-780[R]
Remark BC-6D and SC-CAL PLUS can cover ESR parameter.

8.

Product parameters
The number of measuring parameters
Sample type Model
Reportable
parameter
RUO
parameter
Histogram
68
4
54
4
11
2
2
11
2
2
Scatter diagram
Total 41
WBC group: 13
BC-760[R]
RET group: 7
BC-780[R]
RBC group: 10
7
(Three of them are 3D graphics)
PLT group: 10
Whole
blood
ESR: 1
Total 33
WBC group: 13
BC-760[B]
RBC group: 10
PLT group: 9
4
Two of them are 3D graphics
ESR: 1
BC-760[R] Total 7 WBC group: 6
Body Fluid
BC-780[R]
BC-760[B]
RBC group: 1
Total 7 WBC group: 6
RBC group: 1

9.

Reportable parameters (Whole blood)
Group
WBC
(13)
RET
(7)
Name
White blood cell count
Basophil count
Basophil percentage
Neutrophil count
Neutrophil percentage
Eosinophil count
Eosinophil percentage
Lymphocyte count
Lymphocyte percentage
Monocyte count
Monocyte percentage
Immature granulocyte
Immature granulocyte percentage
Reticulocyte percentage
Reticulocyte count
Reticulocyte
hemoglobin
expression
Immature reticulocyte fraction
Low fluorescent ratio
Middle fluorescent ratio
High fluorescent ratio
Abbreviation Applicable Model
WBC
Bas#
Bas%
Neu#
Neu%
Eos#
Eos%
Lym#
Lym%
Mon#
Mon%
IMG#
IMG%
RET%
RET#
RHE
IRF
LFR
MFR
HFR
General
Group
RBC
(10)
BC-760[R]
/BC-780[R]
PLT
(10)
ESR
(1)
Name
Abbreviation
Red blood cell count
Hemoglobin concentration
Mean corpuscular volume
Mean corpuscular hemoglobin
Mean corpuscular hemoglobin
concentration
Red blood cell distribution width
coefficient of variation
Red blood cell distribution width
standard deviation
Hematocrit
Nucleated red blood cell number
Nucleated
red
blood
cell
percentage
Platelet count
Platelet count- impedance
RBC
HGB
MCV
MCH
Applicable
Model
MCHC
RDW-CV
General
RDW-SD
HCT
NRBC#
NRBC%
PLT
PLT-I
General
Platelet count hybrid
PLT-H
General
Optical platelet count
PLT-O
BC-760[R]
/BC-780[R]
Mean platelet volume
Platelet distribution width
Plateletcrit
Platelet-large cell ratio
Platelet-large cell count
MPV
PDW
PCT
P-LCR
P-LCC
General
Immature platelet fraction
IPF
General
Erythrocyte sedimentation rate
ESR
General

10.

Reportable parameters (Body fluid)
Group
Name
Abbreviation Applicable Model
WBC
(6)
White blood cell count-body fluid
Total nucleated cell count-body fluid
Mononuclear cell count
Mononuclear cell percentage
Polymorphonuclear cell count
Polymorphonuclear cell percentage
WBC-BF
TC-BF#
MN#
MN%
PMN#
PMN%
RET
(1)
Red blood cell count-body fluid
RBC-BF
General

11.

Analysis mode
Sample type
WB
Whole
blood
Analysis mode
CBC, CD, ESR, CBC+ESR, CD+ESR
CBC, CD, Ret, CR, CDR, ESR, CBC+ESR, CD+ESR, CDR+ESR
CD/WBC-3X
Applicable Model
BC-760[B]
BC-760[R]/BC-780[R]
BC-760[B]
Low WBC CD/WBC-3X, CDR/WBC-3X
BC-760[R]/BC-780[R]
Low PLT CR/PLT-5X, CDR/PLT-5X
BC-760[R]/BC-780[R]
Predilute
Body fluid
CBC, CD
BC-760[B]
CBC, CD, Ret, CR, CDR
BC-760[R]/BC-780[R]
CD
BC-760[B]
BC-760[R]/BC-780[R]

12.

Sample volume
Sample type
Analysis mode
Sample volume(μl)
WB
CD
CDR
ESR
CD+ESR
CDR+ESR
CD/WBC-3X
CDR/WBC-3X
25
33
140
160
160
37
42
CR/PLT-5X,CDR/PLT-5X
33
CBC,CD
Ret,CR,CDR
20
20
CD
85
Low WBC WB
Low PLT WB
Predilute
Body fluid

13.

Throughput
Sample type
Whole
blood
WB
Low WBC
Low PLT
Predilute
Body fluid
Analysis mode
Throughput (t/h)
CD
ESR
CD+ESR
CDR
CDR+ESR
CD/WBC-3X
CDR/WBC-3X
CR/PLT-5X CDR/PLT-5X
CBC CD
Ret CR CDR
CD
80
50
40
45
30
60
40
35
50
33
50

14.

15.

Analysis Flowchart

16.

SF CUBE Cell Analysis Technology
The cell size difference can be used to characterize the forward scattered light signal, the intracellular
particle complexity difference can be used to characterize the side scattered light, and the fluorescent
signal intensity reflects the degree of staining of the cell.
The DIFF channel differentiates the major white blood cell subsets (lymphocytes, monocytes, neutrophils,
eosinophils and basophils) by identifying the differences in three-dimensional signals of the cells processed by
the reagent, and identifies and alarms abnormal cells such as immature granulocytes, abnormal lymphoblasts,
and blasts, and counts WBCs at the same time.

17.

DIFF/RET通道检测原理-激光散射鞘流法+荧光染色检测技术

18.

The structure of Optical System
SFL
SSC
FSC
Power
19
Signal
Signal
18
1
17
Power
20
Signal
3
Power
4
5
6
PD
Signal
14
13
15
16
PD
8
11
2
9 10
7
Power
12

19.

DIFF/RET
• Scatter diagram

20.

Confidential
DIFF Channel
HFC
MON IMG
LYM
NEU
BASO
Ghost
20
LD
360° Rotation
FD
EOS

21.

Confidential
RET Channel
360° Rotation
RBC
RET
RBC
RET
PLT
PLT
FR
DR
IPF
21

22.

RET Channel

23.

RBC/PLT Channel _ Sheath fluid impedance method

24.

HGB Channel _ Colorimetric method

25.

Principle of PLT-H

26.

PLT-H _ Hybrid Channel PLT
Normal sample
Abnormal sample
RBC
RBC
PLT False High
Unclear separatrix
Clear separatrix
Small RBCs
RBC
Fragment
PLT
PLT
Volume
Volume
PLT False Low
Large PLTs
PLT
Aggregation
PLT (volume > 10 fL) is easily be
interfered in impendence method.
Solution
After LD Lyse treatment, the structure of PLT is intact.
After LD Lyse treatment, detecting PLT organelle volume.
After LD Lyse treatment, it can bring hemolysis of RBC.

27.

PLT-H _ Hybrid Channel PLT
After LD Lyse treatment
membrane proteins are
lost and membrane pores
are formed.
After LD Lyse treatment
LD Lyse go into the
interior of cell.
RBC Volume
Frequency
PLT
The structure
of PLT is intact.
PLT Volume
After LD Lyse
treatment
RBC
Volume(fL)
Hemolysis
of RBC
⚫ After LD Lyse treatment:
◆ The maximum volume of RBC is about 7.5fL
◆ RBC Fragment will not affect the PLT (volume >
10fL) count .

28.

PLT-H _ Hybrid Channel PLT

29.

30.

Principle of ESR

31.

Easy-W ESR
The analyzer uses Dynamic Capillary Photometer Method to
measure the aggregation degree of red blood cells within the
specified time and calculate the sedimentation distance. The red
blood cells in the whole blood sample will first gather from the
dispersed state to form a coin like structure, and then gather into
a mass and begin to settle. The settling speed mainly depends
on the degree of aggregation. The change of erythrocyte
aggregation state will lead to the change of its scattering effect
on the measurement beam, which is specifically manifested in
that its light transmittance increases with the increase of
aggregation degree. Therefore, the aggregation degree of red
blood cells and the sedimentation rate of red blood cells can be
obtained by measuring the change of light transmittance of
whole blood samples with time

32.

Easy-W ESR
时间
魏氏法

33.

Easy-W ESR
• Erythrocyte aggregation speed determines
the Start Time of Sedimentation Stage:
The faster the aggregation speed, the shorter the
time required for aggregation, the earlier the
sedimentation stage will come, and the higher the 1hour ESR result will get;
• The degree of erythrocyte aggregation
determines the erythrocyte sedimentation
speed:
The aggregation degree of red blood cells
determines the size of cell clusters, which also
determines the sedimentation speed of cell clusters
in the sedimentation stage.
Easy-W ESR:
By measuring the speed and degree of red blood cell
aggregation in a short time, it will get the 1-hour ESR
result highly correlated with Westergren method.

34.

Easy-W ESR
• Measurement flow
RBC depolymerization in ESR
module
Measuring
Erythrocyte
aggregation
Algorithm
The range of curve changing—— Aggregation degree
The rising time of curve —— Aggregation speed
Curve absolute horizontal —— HCT
ESR
Result

35.

36.

37.

BC-760 Appearance

38.

Appearance
正视图
Front View
1
2
3
No.
Description
1
Touch screen
2
Front cover
3
Autoloader
(6 rows of 5-position tube racks)

39.

Appearance
Back View
No.
1
Description
Fans of PCBA boards
2
Fan of main unit
3
Power switch
4
Power socket
5
Waste sensor socket
6
Waste connector
7
8
9
DS Diluent connector
ESR Cleanser connector
LH Lyse connector
10
LD Lyse connector
11
13
DR Lyse connector
LS Lyse connector (only for
CRP/SAA model)
USB 3.0 socket
14
USB 2.0 socket
15
Network cable socket
12

40.

Appearance
Left View
No.
Description
1
USB 2.0 socket
2
Dye replacing compartment
door

41.

Appearance
Right View
No.
Description
1
Power supply key
• Short press for startup.
• Press and hold about 5
seconds for quick
shutdown (Abnormal
shutdown).

42.

Appearance _ Remove Panels

43.

Appearance _ Remove Left & Right Panel

44.

Appearance _ Open the Right Pool Assembly
Right View

45.

Appearance _ Remove Back Panel

46.

Appearance _ Remove Back Panel

47.

Appearance _ Remove Top Panel

48.

BC-760 Mechanical Structure

49.

Mechanical Structure
Front View
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Description
Dye diaphragm pump assembly
Fluid pressure detection assembly
Fluidic Junction module
Dye bag assembly
250uL Syringe assembly
10ml Syringe assembly
Ambient temperature sensor
Loudspeaker
Tube holding assembly
Scanner
Rotary compressing assembly
Sample mixing assembly
Sampling assembly
Pressure detect board

50.

Mechanical Structure
Front Cover
No.
1
2
3
4
Description
PCBA board RFID reader
Startup/shutdown key board
USB 2.0 socket
Screen assembly

51.

Mechanical Structure
Right View
No.
1
2
3
4
5
6
7
8
Description
Sheath flow impedance
measurement assembly
Right side pools assembly
HGB assembly
Reaction bath assembly
Valves for preparing sample of
Optical system
Valves for preparing sample of
Sheath flow impedance
measurement assembly
Valve and Diaphragm pump for
ESR channel
Optical system

52.

Mechanical Structure
Left View
No.
1
2
3
4
5
6
7
8
9
10
11
Description
Isolation chamber
Waste pump assembly
Valves
Air tank assembly
Degassing unit (only for CRP/SAA
model)
Liquid detect board
Sheath filter assembly
Diluent pre-heater assembly
Valves
Waste chamber
Filter assembly of probe wipe

53.

Mechanical Structure
Top View
No.
1
2
3
4
5
6
Description
Main control board
Mother board
Positive pressure pump
Optical sensor
Motor driver board
Valve driver board

54.

Mechanical Structure
Back View
No.
Description
1
Power supply assembly
2
Fans of PCBA boards
3
Fan of main unit
4
Reagent & Waste connectors
5
0.5mL Diaphragm pump

55.

Mechanical Structure
Autoloader (6 rows of 5-position tube racks)
Unloading
mechanism
Horizontal feeding
mechanism
Loading
mechanism
• 5 tube positions per tube rack,
total 6 tube racks can be placed
on the loading mechanism.

56.

Mechanical Structure
Autoloader (6 rows of 5-position tube racks)
Peripheral blood sample
mixing assembly (Optional)
Unloading home position sensor
Left counter sensor
Right counter sensor
Left feeding home
position sensor
Right feeding home position sensor
Loading in-position
micro-switch
Reflective Sensor
Tube rack detection sensor
(transmitter)
Loading home position sensor
Autoloader board PCBA
Loading end position sensor
Sample compartment
home position sensor
Tube rack detection sensor (receiving end)

57.

Mechanical Structure
Autoloader - Sample compartment assembly
Closed-tube
WB position
Position switch
Micro tube
position

58.

Mechanical Structure
Autoloader - Sample compartment assembly
1
3
• The adapter with the
larger-diameter end
upward.
• There is a adapter
into the Micro
Tube Position.
• Without adapter,
it supports for
long micro tube.
2
• The adapter with the
smaller-diameter
end upward.

59.

Mechanical Structure
Sample mixing assembly
No.
1
2
3
4
5
6
7
8
9
Description
Z-axis direction mixing motor
Z-axis direction mixing synchronous belt
Tube pressing plate
Mixing gripper
Gripper piece
R-axis direction mixing synchronous belt
R-axis direction mixing motor
Y-axis direction mixing motor
Y-axis direction mixing synchronous belt

60.

Mechanical Structure
Sampling assembly
√Auto-sampling position
• √ means:
It requires to adjust
mechanical position after
replacing related assembly.
√ Closed-tube
WB position
Micro tube position
RET bath
DIFF bath
HGB bath
√
√
√

61.

Mechanical Structure
Tube compressing
试管压紧装置
assembly
Rotary compressing assembly & Scanner
Tube rotation
试管旋转装置
assembly
Tube
rack
试管架
条码扫描仪
Scanner
Tube
试管

62.

Mechanical Structure
Holding assembly
• To fix the tube in the tube rack
during piercing.

63.

64.

Hardware block diagram

65.

Composition and Functions of Hardware System
No.
1
2
FRU Name
Power Supply Assembly
IMX8 Main Control Board
Function description
Remarks
Supply power to the analyzer, mainly include the 24V/400W power supply.
The fan for power supply
assembly is driven by the
motherboard.
Provide various ports including USB port, network port, hard disk interface and
screen assembly interface; Analog/digital conversion and digital processing of
optical signals, impedance signals, HGB signals and analyzer status monitoring
/
signals;
Convert the +24V output by the 600W power supply to P24V, P12V, D5V, analog +/12V, and analog +/-5V, and provide the power for different boards; Analog/digital
3
Mother Board
conversion and digital processing of optical signals, impedance signals, HGB signals
and analyzer status monitoring signals; Drives motors, pumps, heatings, fans and /
other power components; Detect temperature
sensors, hydraulic sensors, dye sensors, floaters and optocouplers, etc. Interfaces
for level board, air pressure board, valve driver board and ESR assembly, etc.
4
One-glass solution TFT 12.1" Display function and the touch function are provided, including display and
/
1280*800
capacitive touch screen. Connect display assembly to main control board.
5
Liquid detection board
Detect diluent, lyse and the reagents of 6 channels. Output to motherboard;
/

66.

Composition and Functions of Hardware System
No.
FRU Name
Function description
Remarks
6
+50A, +40a, +90 kPa, -30 kPa and -40 kPa, output analog voltage signal
Air pressure detection board
(digital conversion on driver board) Built-in EEPROM, storage pressure /
PCBA
calibration factor .
7
PCBA, RFID Reader PCBA
With RFID antenna board, allow to read and write CRP, SAA emulsion RFID label;
/
Serial communication with main control board;
8
Startup / shutdown key board
Provide physical keys for user input operation, detect the on/off state of keys and
/
control the on/off status of analyzer; The keys are equipped with indicators;
9
Indicator Board PCBA
Provides the analyzer status indication, including the RGB tricolor indicator array
/
and its driver.
10
PCBA, impedance signal board The drive and impedance signal amplification of the counting bath sensor;
/

67.

Category and Purpose of Power Supply
Power supply
Symbol
Power supply
description
Power supply range Purpose
P24V
Power+24V
22.8~25.2V
Motor drive, temperature control heating
P12V
Power+12V
11.5~12.5V
Driver of valve/pump/ board fan and
optical system fan
D12V
Digital+12V
11.5~12.5V
Power supply for main control board,
display
Power supply for digital circuits of
D5V
Digital+5V
4.75~5.25V
motherboard, liquid detection board,
indicator board, RFID reader board; sensor
power supply
Power supply for digital circuits of
D3V3
Digital+3.3V
3.1~3.5V
motherboard, air pressure detection board,
touch screen control .

68.

Category and Purpose of Power Supply
Power supply
Symbol
Power supply
description
Power supply range Purpose
A+12V
Analog+12V
11.5~12.5V
A-12V
Analog-12V
-11.5~-12.5V
A+5V
Analog+5V
4.75~5.25V
A-5V
Analog-5V
-4.75~-5.25V
Power supply for analog circuits of
impedance analog board, optical signal
board, laser drive board, laser control
board.
Power supply for analog circuits of
impedance analog board, optical signal
board, laser drive board, laser control
board.
Power supply for analog circuits of
motherboard, optical signal board and air
pressure detection board.
Power supply for motherboard and optical
signal board.

69.

Temperature control system
Required
Ambient
Target
Temperature (℃) Temperature (℃)
Temperature (℃)
Assembly
Object
Evaluation
point
Diluent
preheating
assembly
Diluent flowing in
the instrument
Preheating bath
Temperature
sensor
Track ambient
temperature
/
15-35
DIFF lyse
Sample for DIFF
batch reaction
42.5
42-43.5
15-35
RET diluent
Sample for RET
batch reaction
42.5
42-43.5
15-35
Reaction bath
assembly

70.

71.

72.

Main Screen

73.

iHelp Video
⚫ The screen will
display the silent
video with English
subtitles.

74.

Menu-Status-Temp.&Pressure

75.

Menu-Status-Floater Status

76.

Menu-Status-Sensor

77.

Menu-Status-Sensor

78.

Menu-Status-Sensor

79.

Menu-Status-Sensor

80.

Menu-Status-Voltage & Current

81.

Menu-Status-Baseline Status

82.

Menu-Setup-Auxiliary Setup

83.

Menu-Setup-Auxiliary Setup
Use DS or Distilled Water instead of DR and FR.
Use DS or Distilled Water instead of ESR Cleanser.

84.

Menu-Setup-Parameter unit setup

85.

Menu-Setup-Para. Setup-Ref. Range Setup

86.

Menu-Setup-Para. Setup-Microsco. Para. Setup

87.

Menu-Setup-Maintenance

88.

Menu-Setup-Reagent Setup

89.

Menu-Setup-System Setup-Barcode

90.

Menu-Setup-System Setup-Communication
Only when "ACK Synchronous Transmission" is selected
can the "Auto Communicate" function be enabled.

91.

Menu-Setup-Gain Setup
• “Admin” access can go to
this interface and click “Auto
Set HGB Gain”.

92.

Menu-Setup-Auto Startup/Shutdown

93.

Table Review-Graph-Special Info.

94.

Menu-Calibration-Manual
It can help the operator quickly
understand the transmission relationship.

95.

Menu-Calibration-Manual
It can help the operator quickly
understand the transmission relationship.

96.

Menu-Calibration-Gain Calibration-CBC Gain Calibration
• There are differences in
the detection sensors of
CBC channel.
• The service engineer
needs to eliminate the
differences by adjusting
the gain to make the
consistency of the
machine meet the
requirements.

97.

Menu-Calibration-Gain Calibration-Optical Gain Calibration
• The service engineer
needs to eliminate the
differences by
adjusting the gain to
make the consistency
of the machine meet
the requirements.

98.

Menu-Calibration-ESR Calibration
Please use SC-CAL Plus Calibrator.
Level
Dilution ratio
Plus DS
Diluent
Description
Low
1mL 0
ESR
Normal
1mL 1ml
ESR(1:1)
High
1mL 2ml
ESR(1:2)
Enter the target value in the order of low
value → normal value → high value, and
measure it three times.
Only required after replacing or opening
ESR module .

99.

Menu-Service-Debug & Self-test –Adj. Sample Probe Pos.

100.

Menu-Service-Debug & Self-test –Adjust Autoload Pos.
• Mixing gripper adjustment.
• Left/Right feeding pos.
needs a special tooling for
autoloader adjustment in
factory.
Normally engineer doesn’t
need to adjust it at
customer site.

101.

Menu-Service-Debug & Self test-Heating debug
• Record and export the Temperature Monition Curve of 5 channels.

102.

Menu-Service-Debug & Self test-Pressure debug
• Generate the pressure, then record and export the Pressure Curve of 3 channels.

103.

Menu-Service-Debug & Self test-Sensor debug
• Click “Calibrate”, and
follow the prompts to
confirm that the gain
value of the dye sensor
and the detection
voltage are within the
range.
Then click “Save”.

104.

Menu-Service-Debug & Self test-Sensor debug
• Set the appropriate LED
current value and save it.
• “ESR background
intensity” will change
accordingly, and confirm
that the set value of “ESR
background intensity” is
within the range.
• The higher the LED
current value, the lower
the ESR background
intensity.

105.

Menu-Service-Debug & Self test-Sensor debug
• If the position of the
measurement point is
not within the range,
click “Calibrate” and save.

106.

Menu-Service-Advanced toolbox

107.

Menu-Service-Advanced toolbox
Clear Data:
You can clear the test
records, sample
information, etc. with one
click, and retain the
calibration coefficient and
gain coefficient

108.

Menu-Service-Advanced toolbox-Debug Data Export
Maximum storage 3500 INF files.

109.

Menu-Service-Precise Fault Diagnosis
• Some errors cannot be eliminated by one
click, and there are many associated FRU
items. You can come to the “Precise Fault
Diagnosis" interface for further accurate fault
location analysis. The instrument
automatically runs the self-check sequence
and gives the diagnosis conclusion.
Error Description
HGB blank voltage
abnormal
50kPa pressure out of
range
-40kpa pressure out of
range
Precise Fault Diagnosis 40kpa pressure out of
range
Preheating bath priming
abnormal
Cistern floater abnormal
Error Code
30201
10300
10304
10302
10100
10101

110.

Menu-Status-Version Info.

111.

112.

Mechanical debugging

113.

Adjustment of sample probe position
• Vertical direction home
pos.
1. Go to “Menu”>“Service”>“Debug
& Self-Test”>“Adj. Sample Probe
Pos.” interface.
2. Select “Vertical direction home
pos.”.
3. Click “To Default Adjust Pos.”.

114.

Adjustment of sample probe position
4. Click the “Down” button to adjust the
sampling probe to be flush with the
bottom of the probe wipe.
5. After finishing, click “Save steps” the
number of steps, and then click the
“Observe” button to confirm that the
adjustment position is correct (The
probe tip is flush with the bottom of the
probe wipe), and then click “Exit” the
observation.
If the position is not correct, repeat the
above adjustment steps.
A
Sampling Probe
B
Probe wipe
C
The probe tip is flush with the
bottom of the probe wipe

115.

Adjustment of sample probe position
• Reaction bath home pos.
(Same with DIFF bath pos.)
1. Go to “Menu”>“Service”>“Debug &
Self-Test”>“Adj. Sample Probe Pos.”
interface.
2. Select “Reaction bath home pos.”.
3. Click “To Default Adjust Pos.”.
DIFF
RET
Default Adjust Pos.
DIFF
RET

116.

Adjustment of sample probe position
4. Click the “Forward”, “Backward”, “up”, and
“down” buttons to adjust the front and rear
positions and vertical height of the sampling
probe (the vertical height position can be
confirmed with a piece of paper).
DIFF
RET
Requirements: The sampling probe in the
horizontal direction is centered from the bath
walls, and the probe tip in the vertical direction
is just in contact with the upper end face of
the bath wall.
DIFF
5. After the adjustment is completed, click “Save
steps”.
RET

117.

Adjustment of sample probe position
6. Click “Observe” button, the sampling probe
will move horizontally to the horizontal center
position of RET bath, and the probe tip will
move vertically to the position of the top
surface of the bath.
DIFF
RET

118.

Adjustment of sample probe position
7. Click “Verify” button to confirm that the
adjustment position is correct, and then click “Exit”.
Note: After the “Verify” button is pressed, the
sampling probe will reach the last position of the
count, at this time the sampling probe does not
interfere with the bath wall.
DIFF
RET

119.

Adjustment of sample probe position
• HGB bath pos.
1. Remove the shield cove of
HGB bath.
2. Go to
“Menu”>“Service”>“Debug &
Self-Test”>“Adj. Sample
Probe Pos.” interface.
3. Select “HGB bath pos.”.
4. Click “To Default Adjust Pos.”.
Default Adjust Pos.

120.

Adjustment of sample probe position
5. Click the “Forward”, “Backward”, “up”, and
“down” buttons to adjust the front and rear
positions and vertical height of the sampling
probe (the vertical height position can be
confirmed with a piece of paper).
Requirements: The sampling probe stops at
the upper left part of the bath hole, the
sampling probe is horizontally aligned in the
center of the bath, and the probe tip is flush
with the top surface of the bath wall.
6. After the adjustment is completed, click “Save
steps”.

121.

Adjustment of sample probe position
7. Click “Verify” button to confirm that the
adjustment position is correct, and then click “Exit”.
Note: After the “Verify” button is pressed, the
sampling probe will reach the last position of the
count, at this time, the piercing probe tip is within
the range of the HGB spot and does not interfere
with the bath wall.

122.

Adjustment of sample probe position
• CT WB pos.
1. Go to “Menu”>“Service”>“Debug &
Self-Test”>“Adj. Sample Probe Pos.”
interface.
2. Select “CT WB pos.”.
3. Click “To Default Adjust Pos.”.

123.

Adjustment of sample probe position
4. Click Up, Down, Forward, and Backward
until the probe tip is level with the upper
surface of the sample compartment and the
probe is aligned with the arrow on the label
in the horizontal direction (Position 1, as
shown in the figure).
5. To adjust the upper and lower positions,
put an A4 paper between the probe tip and
the upper surface of the sample
compartment. Move the paper, and it can
be dragged. If it feels like pulling, adjust the
upper and lower positions in place.
6. Click "Save Steps" > "Ok" > "Exit" to
complete the adjustment.

124.

Adjustment of sample probe position
• Auto sampling pos.
1. Go to “Menu”>“Service”>“Debug &
Self-Test”>“Adj. Sample Probe Pos.”
interface.
2. Select “Auto sampling pos.”.
3. Click “To Default Adjust Pos.”.

125.

Adjustment of sample probe position
4. Click Up, Down, Forward, and Backward
until the probe tip is level with the floating
blood remover, and the center of the
sample probe is aligned with the notch in
the horizontal direction.
5. To adjust the upper and lower positions,
put an A4 paper between the probe tip and
the floating blood remover. Move the paper.
If the paper can be dragged, adjust the
upper and lower positions in place.
6. Click "Save Steps" > "Ok" > "Exit" to
complete the adjustment.

126.

Adjustment of sample probe position
• Note:
If you forget the standard of sample
probe position adjustment, click
“Help” button for detailed
adjustment information.

127.

Adjustment of Rotary compressing assembly
1. Click "Menu" > "Service" >
"Debug&Self-Test" > “Self-test“, and
select tab "Autoloading Assembly" to
enter the autoloading adjustment
screen.
2. Place the WB tube rack with tube in
position 1 on the autoloader.
3. Click “Initialization” firstly, then click
"Loading" > "Feeding" to move the
tube to the corresponding position of
the rotary compressing assembly.

128.

Adjustment of Rotary compressing assembly
4. Click “Fix Tube," and observe whether
both of compressing wheels against
the tube properly.
Whether rotary wheel against the
tube properly.
If the tube rack is moved, or
compressing wheel does not close to
the tube, click "Release Tube.“ and go
to step 5.
Otherwise, click "Release Tube.“ and
finish the adjustment.
Left compressing
wheel
Right compressing
wheel

129.

Adjustment of Rotary compressing assembly
5. Unscrew these 4 screws to adjust
the horizontal left and right
position, and tighten the screws.
Then go back to step 1~4.

130.

Adjustment of Scanner position
1. Click "Menu" > "Service" >
"Debug&Self-Test" > “Self-test“, and
select tab "Autoloading Assembly" to
enter the autoloading adjustment
screen.
2. Click “Initialization” firstly, then click
“Scanner On” and check if the
scanner is turned on normally.
Click “Scanner Off” and check if the
scanner is turned off normally.

131.

Adjustment of Scanner position
3. Place the rack with tubes on the autoloader.
4. Click "Auto Read Barcode" to see if the result
is correct. If not, proceed to the next step.
5. Unscrew these 2 screws to adjust the
horizontal left and right position, and tighten
the screws.

132.

Adjustment of mixing gripper position
• Mixing gripper
(front position)
1. Go to “Menu”>“Service”>“Debug
& Self-Test”>“Adjust Autoload Pos.”
interface.
2. Select “Mixing gripper (front
position)”.

133.

Adjustment of mixing gripper position
3. Place an empty rack on the
autoloader.
4. Click “Check by step“, and click
“Feeding” to let the # 1 position
of the tube rack is at the mixing
position, then click “Finish”.

134.

Adjustment of mixing gripper position
5. Loosen these 2 screws of gripper.
Click “To Adjust Pos.” and observe that if the
mixing gripper is upright or not.
If not , click “Left” and “Right” to adjust rotation
direction.
Then tighten the screws.

135.

Adjustment of mixing gripper position
6. Unscrew these 4 screws to adjust
the horizontal left and right
position of Sample Mixing
Assembly.
Make sure the gripper is at the
center of tube position in left and
right direction, then tighten the
screws.

136.

Adjustment of mixing gripper position
7. Click “Forward” and “Backward” to adjust the
horizontal forward and backward position of
gripper.
Make sure the line-1 and line-2 is coincided.

137.

Adjustment of mixing gripper position
8. Click “Up” and “Down” to adjust the vertical up
and down position of gripper.
Make sure the distance between gripper and rack
is 1.5~2 mm.
Click “Save Steps” and “Exit”.

138.

Adjustment of mixing gripper position
8. Verification:
①Place a tube rack with tubes
on the autoloader.
②Click “Check by step” and
“Feeding” to let the # 1
position of the tube rack is at
the mixing position.
③Click “A&M”, and observe
that if the gripper will catch the
tube to mix and then put it
back to the rack normally.

139.

Gain debugging

140.

Gain debugging
Content
Debugging timing and impact
Optical gain
It affects the WBC scatter diagram and DIFF results.
CBC gain
It affects the RBC and PLT histogram and results.
It needs to debug the gain firstly, then calibration.
HGB gain
When the instrument indicates that the HGB voltage is abnormal, adjust
the gain to the normal range.

141.

Optical gain calibration
1. Go to “Menu”>“Calibration”>“Gain
calibration”>“Optical gain calibration”
interface.
2. Select “Calibrator” and enter the optical
target of calibrator in the target column.
3. Prepare the calibrator and click “Start
Count”.
4. After measurement, a prompt of saving
current count appears.
In case of normal counting, click “Yes”
and continue the following count.
5. After continuous measurement, if it is
≤2.0% in the deviation column.
Click “Save” to save the data and record
the gain factor in the table.

142.

Optical gain calibration
6. Go to “Menu”>“Setup”>“Gain
setup” interface to check whether
the Set Value of optical gain has
been updated.
7. Nnormally the PMT gain value
does not need to be changed
manually, but if the target gain
value of FL channel exceeds the
range, it is necessary to change
the PMT gain value manually and
restart testing.

143.

CBC gain calibration
1. Go to “Menu”>“Calibration”>“Gain
calibration”>“CBC gain calibration”
interface.
2. Enter the MCV_G target value for the
batch of calibrator used.
3. Perform counting continuously until “OK”
is displayed in the result columns of
both MCV_G and HGB.
4. When exiting the CBC Gain screen, click
“Yes” on the pop-up dialog box, save
the gain and complete the
commissioning process.

144.

HGB gain debugging
• There are 2 methods for HGB gain
debugging:
① Method 1
Refer to the CBC gain calibration
above. If OK is displayed in the HGB
column, the debugging is complete.
② Method 2
Click “Menu”>“Setup”>“Gain setup” to
enter the Gain Setup screen.
Click “Auto set HGB gain” until the
value in the HGB blank voltage
column is within the range.
Exiting the “Gain Setup” screen, click
“Yes” on the pop-up dialog box,
save the gain and complete.

145.

146.

Function of Fluidic Parts

147.

Solenoid Valve
• Two-way solenoid valve
• Function:
Build or cut a passage. The two-way valve includes normally closed two-way valve and normally open two-way
valve. When the normally closed valve is not energized, the input and output ends are disconnected. When the
valve is energized, the input and output ends of the normally closed valve are connected. The normally open valve
and the normally closed valve result in the difference results after being energized. The analyzer uses the normally
closed two-way valve only.
• Three-way solenoid valve
• Function:
Switch among passages. When the three-way valve is not energized, the common end (COM) and the normally
open end (NO) are connected. When the three-way valve is energized, the common end and the normally closed
end (NC) are connected.

148.

Pressure-proof Solenoid Valve
• Function:
Compared with ordinary solenoid valves, pressure-proof valves are used for diluent channels, with a pressure
range greater than and the working principles same as those of ordinary solenoid valves.
The label for an ordinary valve is grayish, and the marked pressure-proof parameter is 0.2 MPa, while the label
for a pressure-proof valve is whiter, and the marked pressure-proof parameter is 0.25 MPa.

149.

SMC Valve
• Function:
Same with solenoid valve, the analyzer mainly use the SMC valve.
SMC valve is also divided into two-way SMC valve and three-way SMC valve. They are completely consistent in
appearance that all look like three-way valve, it only can be distinguished by the model information on the valve body.
• Model:
1. The model LVM10R3-6A-2-Q is a two-way SMC valve, and the available nozzle numbers are port "1" and "2“.
2. The model LVM10R6-6A-2-Q is a two-way SMC valve, and the available nozzle numbers are port "1" and “3“.
3. The model LVM105R-6A-2-Q is a three-way SMC valve, and the available nozzle numbers are port "1" , “2” and “3“.

150.

Syringes
Name
Diluent syringe
Sample injection
syringe
Label
DIL_SYRINGE
SP_SYRINGE
DIL_Syringe
Specifications
Functions
10mL
Clean the sampling probe, HGB bath and optical
channel, prime the SCI bath, and supply the diluent to
the reaction bath and the sheath fluid to the flow cell.
250uL
Aspirate and distribute the blood samples of specified
volume, depolymerize the ESR samples, dispense the
sample to the flow cell and measure in the sheath fluid
impedance count bath.
SP_Syringe

151.

Pumps
Name
Label
Specifications
Functions
Gas pump
GP
Positive pressure
Provide a pressure source for the pneumatic system,
and use compressed air to drive the fluidics.
Pump_VAC
Vacuum pressure
Collect and drain the wipe waste, reaction bath waste
and RBC sample preparation tube waste.
Pump_WASTE
Vacuum pressure Empty the waste cisterns WC2.
Waste pump
Gas pump
Waste pump

152.

Dosing Pump
• Function:
Control the reagent dosing.
• Model:
1. DP1: 0.5mL dosing pump; Dispense the DR diluent of specified volume to the RET bath.
2. DP2: 0.5mL dosing pump; Dispense the LD lyse of specified volume to the DIFF bath.
3. DP3: 0.5mL dosing pump; Dispense the LH lyse of specified volume to the HGB bath.
4. DP4: 20uL dosing pump; Dispense the FR dye of specified volume to the RET bath.
5. DP5: 20uL dosing pump; Dispense the FD dye of specified volume to the DIFF bath.
6. DP6: 0.5mL dosing pump; Dispense the ESR Cleanser of specified volume to the RET bath.
0.5mL dosing pump
20uL dosing pump
(in Dye dosing pump assembly)

153.

Filters
Name
Label
Diluent filter
LF1
Filter the impurities in the diluent, protect the optical channel,
flow along the direction of letters.
LF2
Filter the impurities from the HGB bath, protect the solenoid valve
SV09.
LF3
Filter the impurities from the RET bath, protect the solenoid valve
SV06.
LF4
Filter the impurities from the DIFF bath, protect the solenoid valve
SV07.
LF8
Filter the impurities from the sheath fluid impedance assembly,
protect the waste pump PUMP_VAC.
Sheath fluid filter
LF5
Intercept tiny bubbles in the optical sheath fluid channel.
Probe Wipe Filter
LF6
Filter the impurities from the probe wipe, protect the solenoid
valve SV08 and waste pump PUMP_VAC.
Waste filter
(the same materials)
Functions

154.

Filters
Waste filter (LF2\LF3\LF4\LF8)
Diluent filter (LF1)
Sheath fluid filter (LF5)
Probe wipe filter (LF6)

155.

Sampling probe & Probe wipe
• Sampling probe
• Probe wipe
• Function:
The sampling probe is used to aspirate and distribute
samples, The sampling probe move between each
incubation bath through horizontal and vertical
motors to distribute samples.
• Function:
Clean the exterior of sample probe, dry and recover
the cleaning waste of sample probe interior and
exterior.

156.

Sampling Pressure Sensor
Name
Label
Functions
Sampling Pressure
Sensor
LPS
Collect the information on the fluid pressure in the sampling tube.

157.

Wearing Parts
Description
Service life
Notes
Piercing probe
120,000 times of piercing or 3 years
It needs to confirm “Vertical direction
home pos.” after replacement.
Probe wipe
120,000 times of piercing or 3 years
It needs to confirm “Vertical direction
home pos.” after replacement.
Probe wipe filter (LF6)
60,000 times of count or 3 years
N/A